Human Cardiac Troponin-T (Native)

Product Code

10588A

Stage / Grade

Highly Purified / Immunogen Grade

R.M. Source

Human Cardiac Tissue

Concentration Range

0.5 - 2.5 mg/mL

Buffer Matrix & pH

6M Urea, 250mM NaCl, 50mM Tris.HCl, 15mM β-mercaptoethanol, 1mM EDTA (pH: 7.2)

Optimal Storage Temperature

-50 °C or ↓ (avoid repeated freeze-thawing)

Quality Control Methods / Results

Protein Quantitation Method

Concentration determined by A280 assuming an extinction coefficient of 16,960cm^-1M^-1 at 280 nm and a molecular weight of 34,575

Protein % Purity Range

≥ 95%

Quality Methods Used

SDS PAGE: Single ~35 kDa band

Physical Characteristics

Shelf Life

5 years @ -50 °C or ↓

Filtration

No filtration

Troponin-T is one of three subunits that form the troponin complex of the thin filaments of striated muscle. Cardiac Troponin-T is characterized by a molecular weight of ~35 kDa. It is expressed only in the heart and has proved to be a specific biomarker for heart disease. cTn-T is purified using a combination of ion-exchange and affinity chromatography steps in urea containing buffers.

HAZARD TESTING & WARNINGS:

Blood samples from tissue donors used in the manufacture of this product tested negative for HBsAg, Anti-HIV-1/2, HIV-1 Ag, HCV Ab, HCV Ag, & RPR/STS by FDA approved methods.

CAUTION: NO TEST CAN GUARANTEE A PRODUCT TO BE NON-INFECTIOUS. THEREFORE, ALL MATERIAL DERIVED FROM HUMAN FLUIDS OR TISSUES SHOULD BE CONSIDERED AS POTENTIALLY INFECTIOUS.

Use Statement: For Research, Remanufacturing, or Immunization use for products not subject to license under PHS Act Section 351 only.