Human Cardiac Tropomyosin

Human Cardiac Tropomyosin
Product Code
10584A
Stage / Grade
Highly Purified
R.M. Source
Human Cardiac Tissue
Concentration Range
0.25 - 2.0 mg/mL
Buffer Matrix & pH
50mM NaCl, 10mM Tris.HCl, 5mM β-mercaptoethanol, 1mM EDTA (pH 7.4)
Optimal Storage Temperature
-20 °C to -70 °C (avoid repeated freeze thawing)

Quality Control Methods / Results

Protein % Purity Range
≥ 90%
Quality Methods Used
SDS PAGE: Single band. Identity confirmed by SDS PAGE mobility shift in the presence of urea (L.B. Smillie, Methods in Enzymology, 85:234-241)
Total Protein Method
Bradford Protein Assay using Bovine IgG as reference standard

Physical Characteristics

Shelf Life
5 years at -25 °C or ↓
Filtration
No filtration

Tropomyosin is an alpha helical coiled protein dimer that binds end to end along F actin filaments in muscle.  It exists in two forms (α and β), the ratios of which vary with muscle type.  It is prepared using a modification of the procedure described by Smillie (above reference).

 

HAZARD TESTING & WARNINGS:

Blood samples from tissue donors used in the manufacture of this product tested negative for HBsAg, Anti-HIV-1/HIV-2, and HCV by FDA approved methods and all results were non-reactive.

 

CAUTION: NO TEST CAN GUARANTEE A PRODUCT TO BE NON-INFECTIOUS.  THEREFORE, ALL MATERIAL DERIVED FROM HUMAN FLUIDS OR TISSUES SHOULD BE CONSIDERED AS POTENTIALLY INFECTIOUS.

 

Use Statement: For Research or Remanufacture of non-injectable products not subject to license under PHS Act Section 351 only.