Human Cardiac Myoglobin

Human Cardiac Myoglobin
Product Code
10580A
Stage / Grade
Highly Purified / Immunogen Grade
R.M. Source
Human Cardiac Tissue
Concentration Range
0.5 - 10.0 mg/mL
Buffer Matrix & pH
50% Glycerol, 150mM NaCl, 10mM sodium phosphate, 0.05% NaN3 (pH 7.0)
Optimal Storage Temperature
-20 °C (do not store at lower temperatures)

Quality Control Methods / Results

Protein % Purity Range
≥ 98%
Quality Methods Used
SDS PAGE: Single 17 kDa band
Western Blot: Identity confirmed using goat anti-human cardiac myoglobin antisera
Total Protein Method
Bradford Protein Assay vs. BSA as reference standard

Physical Characteristics

Color
Slightly Red
Clarity
Clear
Shelf Life
5 years @ -20 °C
Filtration
0.2 μm filtered

Myoglobin is a single-chain heme containing protein with a molecular weight of ~17 kDa.  It is the primary oxygen-carrying protein of muscle tissues.  The same isoform is expressed in both cardiac and skeletal muscle.  It is a sensitive biomarker for muscle injury and has been widely used as a marker of heart disease.  Myoglobin is purified using a combination of gel-filtration and ion-exchange chromatography steps.

 

HAZARD TESTING & WARNINGS:

Blood samples from tissue donors used in the manufacture of this product tested negative for HBsAg, Anti-HIV-1/HIV-2, Anti-HTLV 1/2, anti-HepB core, Anti-Hepatitis C, and RPR/STS by FDA approved methods.

 

CAUTION: NO TEST CAN GUARANTEE A PRODUCT TO BE NON-INFECTIOUS.  THEREFORE, ALL MATERIAL DERIVED FROM HUMAN FLUIDS OR TISSUES SHOULD BE CONSIDERED AS POTENTIALLY INFECTIOUS.

 

Use Statement: For Research, Remanufacturing, or Immunization use for products not subject to license under PHS Act Section 351 only.