Human Cardiac Fatty Acid Binding Protein

Product Code

10570A

Stage / Grade

Highly Purified

R.M. Source

Human Cardiac Tissue

Concentration Range

0.5 - 2.0 mg/mL

Buffer Matrix & pH

150mM NaCl, 10mM sodium phosphate, 0.05% NaN₃ (pH 7.2) -- or -- 75mM NaCl, 5mM sodium phosphate (pH 7.2), 0.025% NaN₃ containing 50% glycerol

Optimal Storage Temperature

-20 °C (do not store at lower temperatures)

Quality Control Methods / Results

Protein % Purity Range

≥ 98%

Quality Methods Used

SDS PAGE: Single 15 kDa band
Western Blot: Identity confirmed with Human cardiac FABP antisera

Total Protein Method

Bradford Protein Assay vs. BSA as reference standard

Physical Characteristics

Shelf Life

5 years @ -20 °C

Filtration

No filtration

Cardiac fatty acid-binding protein (FABP) is a small cytosolic protein that is abundant in the heart and skeletal muscle. Following cardiac muscle injury it appears in the blood as early as 1.5 h, peaks around 6 h, and returns to baseline values in 24 h. These features make cardiac FABP a useful biomarker for detection of heart disease. FABP is purified using a combination of gel-filtration and ion-exchange chromatography.

HAZARD TESTING & WARNINGS:

Blood samples from tissue donors used in the manufacture of this product tested negative for HBsAg, Anti-HIV-1/HIV-2, and HCV by FDA approved methods and all results were non-reactive.

CAUTION: NO TEST CAN GUARANTEE A PRODUCT TO BE NON-INFECTIOUS. THEREFORE, ALL MATERIAL DERIVED FROM HUMAN FLUIDS OR TISSUES SHOULD BE CONSIDERED AS POTENTIALLY INFECTIOUS.

Use Statement: For Research or Remanufacture of non-injectable products not subject to license under PHS Act Section 351 only.